https://dspace.sduaher.ac.in/jspui/handle/123456789/8345 Sat, 04 Apr 2026 01:29:36 GMT 2026-04-04T01:29:36Z https://dspace.sduaher.ac.in/jspui/handle/123456789/9364 Title: Comparison of Haemoglobin Estimation of Blood Donors by Specific Gravity Method, HemoCue Method and Automated Haematology Cell Analyzer Authors: Vyshnavi, V.; Mohiyuddin, S. M. Azeem;; Mohammadi, Kouser Abstract: is fit to donate with the intention of preventing inadvertent donation from an anaemic donor. The aim of the study was to compare the efficacy of the three common Hb estimation methods, namely, copper sulphate (CuSO4) method, HemoCue photometer and automated cell counter in reporting the Hb levels of blood donors. Materials and Methods: CuSo4 specific gravity method, HemoCue and automated cell analyzer (Sysmex XN‑550) were used to determine the Hb levels in blood samples of 500 donors. Descriptive statistics were used to analyse the demographic details of the donor. Kappa statistics were used to determine the level of agreement between the three methods of Hb estimation. Results: HemoCue was found to be more sensitive (86.21%), whereas CuSo4 (97.88%) was found to be more specific. Kappa agreement was good between CuSo4 and Sysmex XN‑550 (0.703), whereas it was moderate between HemoCue and Sysmex XN‑550 (0.458). Conclusions: The CuSO4 method is still viable for Hb estimation among blood donors. Thus, it can be utilised as the primary screening method; however, follow‑up testing with HemoCue or automated cell analyzer can be done to minimise unnecessary deferrals and false acceptance. Thu, 01 Sep 2022 00:00:00 GMT https://dspace.sduaher.ac.in/jspui/handle/123456789/9364 2022-09-01T00:00:00Z https://dspace.sduaher.ac.in/jspui/handle/123456789/9362 Title: Comparison of Haemoglobin Estimation of Blood Donors by Specific Gravity Method, HemoCue Method and Automated Haematology Cell Analyzer Authors: Choudhari, Nikhil; Subhashish, Das; Ravi Kumar Abstract: Introduction: Pre‑donation haemoglobin (Hb) screening is among the foremost test done on blood donors to determine whether an individual is fit to donate with the intention of preventing inadvertent donation from an anaemic donor. The aim of the study was to compare the efficacy of the three common Hb estimation methods, namely, copper sulphate (CuSO4) method, HemoCue photometer and automated cell counter in reporting the Hb levels of blood donors. Materials and Methods: CuSo4 specific gravity method, HemoCue and automated cell analyzer (Sysmex XN‑550) were used to determine the Hb levels in blood samples of 500 donors. Descriptive statistics were used to analyse the demographic details of the donor. Kappa statistics were used to determine the level of agreement between the three methods of Hb estimation. Results: HemoCue was found to be more sensitive (86.21%), whereas CuSo4 (97.88%) was found to be more specific. Kappa agreement was good between CuSo4 and Sysmex XN‑550 (0.703), whereas it was moderate between HemoCue and Sysmex XN‑550 (0.458). Conclusions: The CuSO4 method is still viable for Hb estimation among blood donors. Thus, it can be utilised as the primary screening method; however, follow‑up testing with HemoCue or automated cell analyzer can be done to minimise unnecessary deferrals and false acceptance. Thu, 01 Sep 2022 00:00:00 GMT https://dspace.sduaher.ac.in/jspui/handle/123456789/9362 2022-09-01T00:00:00Z https://dspace.sduaher.ac.in/jspui/handle/123456789/9047 Title: Comparision of Allogenic Cartilage and Autologous Cortical Bone in Ossicular Reconstruction: A Comparative Study Authors: K. C., Prasad; Koneru, Prathyusha; M. B., Swapanthi; P. K., Anjali; Gopi, Indu Varsha Abstract: Ossicular discontinuity is the most common cause of conductive hearing loss. The use of ossicular graft material in ossicular chain reconstruction significantly improves the result in hearing. This study was conducted to compare and analyze the outcome of ossicular reconstruction using allogenic septal spur cartilage and autologous cortical bone in terms of hearing results and graft uptake rates. Study design: randomized clinical trial. Study included 112 patients visiting our ENT department. Patients between 16 and 50 years of age with history of chronic ear discharge and air–bone–gap (ABG) of[35 dB and ossicular involvement were included in the study. The patients underwent detailed ENT examination, audiological and radiological assessment of temporal bone and those patients with evidence of ossicular erosion were subjected to ossiculoplasty with allogenic septal spur cartilage (group I) and autologous cortical bone (group II) randomly. The patients were followed up to 6 months to analyze functional and anatomical results. 50 patients out of 56 patients (90%) from group I who underwent allogenic septal cartilage ossicular reconstruction showed significant improvement in hearing as assessed by pure tone audiogram after 3 months and 6 months. Remaining 10% of patients who did not show hearing improvement on PTA were reopened after 6 months. It was observed that the stapes head got necrosed in them. 40 patients (72%) out of 56 patients (50%) from group II who underwent autologous cortical bone reconstruction showed hearing improvement. Remaining 16 patients (28%) showed no hearing improvement. They were reopened and ankylosis, dislocation of ossicle and extrusion were noted. In our study, graft uptake rates, formation of retraction pockets, and hearing improvements were analyzed. Complications like ankylosis formation, dislocation of ossicle and extrusion rates were more in the group II compared to group I. Hearing results of group I are better compared to group II and the allogenic septal cartilage being readily available is a good option for ossicular reconstruction. Mon, 01 Aug 2022 00:00:00 GMT https://dspace.sduaher.ac.in/jspui/handle/123456789/9047 2022-08-01T00:00:00Z https://dspace.sduaher.ac.in/jspui/handle/123456789/9007 Title: Decision making for treatment of chylous firstula following neck surgery: A challenge to the Surgeon. Authors: Gupta, Arjun; Azeem Mohiyuddin, S.M.; Mohammadi, A Kouser; Babu, Prashanth; N, Harshitha; Thakur., Kunal Abstract: Chylous Fistula following neck surgery is an uncommon but challenging complication encountered by head and neck surgeons. This leads to skin flap necrosis, infection, vascular blow outs and chylothorax. Co-morbidities like malnutrition, diabetes mellitus, radiated neck predispose to chylous fistula. The common site of leak is thoracic duct or lower jugular lymphatics. Identification of chyle leak intra-operatively can be difficult. Chyle leaks more than 500ml/day are termed high output leaks and require re-exploration of the wound and ligation of injured lymphatic duct. Low output leaks settle down with conservative treatment like high protein and low fat naso-gastric feeds, dependent drain, aspiration of collected chyle, antibiotics and octreotide, a somatostatin derivative. Chyle leaks less than 500ml/day and more than 200ml/day are a dilemma in deciding the treatment modality. Chylous fistula not responding to conservative treatment can cause life threatening complications and contaminate or macerate the neck making re-exploration at a later stage difficult. The decision to abandon conservative treatment and the ideal time for it is controversial. We are presenting our experience with the outcome of post-operative chylous fistula in a series of 11 under-nourished patients following neck dissection for malignancies, cystic hygroma and traumatic, contaminated neck injuries. All these patients developed left sided chylous fistula measuring 300ml to 400ml/day, 2 to 4 days after surgery. They were successfully managed by re-exploration of neck and ligation of injured lymphatic duct after 3 to 5 days trial of conservative management. There were no major complications or delay in adjuvant treatment Fri, 01 Oct 2021 00:00:00 GMT https://dspace.sduaher.ac.in/jspui/handle/123456789/9007 2021-10-01T00:00:00Z